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Fig. 6. Blood pressure, kidney function and histology in low-salt with candesartan with or without <t>L-NAME.</t> A. NO production in kidney tissue in 4 groups nephritis rats: normal-salt with candesartan (Dox+Can+NS), low-salt with candesartan (Dox+Can+LS), low-salt with candesartan and L-NAME (Dox+Can+LS+L- NAME), and normal-salt with candesartan and L-NAME (Dox+Can+NS +L-NAME). B-D. Blood pressure included SBP, DBP and MAP in the 4 groups. E-G. Kidney function index included SCr and BUN and calculated Ccr. H. Kidney histology changes in the 4 groups. The black arrowhead indicates glomerular shrinkage, and the yellow arrowhead indicates renal tubular necrosis. Scale bar= 100 µm. n = 4–6/group. * P < 0.05, * * P < 0.01.
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Fig. 6. Blood pressure, kidney function and histology in low-salt with candesartan with or without <t>L-NAME.</t> A. NO production in kidney tissue in 4 groups nephritis rats: normal-salt with candesartan (Dox+Can+NS), low-salt with candesartan (Dox+Can+LS), low-salt with candesartan and L-NAME (Dox+Can+LS+L- NAME), and normal-salt with candesartan and L-NAME (Dox+Can+NS +L-NAME). B-D. Blood pressure included SBP, DBP and MAP in the 4 groups. E-G. Kidney function index included SCr and BUN and calculated Ccr. H. Kidney histology changes in the 4 groups. The black arrowhead indicates glomerular shrinkage, and the yellow arrowhead indicates renal tubular necrosis. Scale bar= 100 µm. n = 4–6/group. * P < 0.05, * * P < 0.01.
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Fig. 6. Blood pressure, kidney function and histology in low-salt with candesartan with or without <t>L-NAME.</t> A. NO production in kidney tissue in 4 groups nephritis rats: normal-salt with candesartan (Dox+Can+NS), low-salt with candesartan (Dox+Can+LS), low-salt with candesartan and L-NAME (Dox+Can+LS+L- NAME), and normal-salt with candesartan and L-NAME (Dox+Can+NS +L-NAME). B-D. Blood pressure included SBP, DBP and MAP in the 4 groups. E-G. Kidney function index included SCr and BUN and calculated Ccr. H. Kidney histology changes in the 4 groups. The black arrowhead indicates glomerular shrinkage, and the yellow arrowhead indicates renal tubular necrosis. Scale bar= 100 µm. n = 4–6/group. * P < 0.05, * * P < 0.01.
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Fig. 6. Blood pressure, kidney function and histology in low-salt with candesartan with or without <t>L-NAME.</t> A. NO production in kidney tissue in 4 groups nephritis rats: normal-salt with candesartan (Dox+Can+NS), low-salt with candesartan (Dox+Can+LS), low-salt with candesartan and L-NAME (Dox+Can+LS+L- NAME), and normal-salt with candesartan and L-NAME (Dox+Can+NS +L-NAME). B-D. Blood pressure included SBP, DBP and MAP in the 4 groups. E-G. Kidney function index included SCr and BUN and calculated Ccr. H. Kidney histology changes in the 4 groups. The black arrowhead indicates glomerular shrinkage, and the yellow arrowhead indicates renal tubular necrosis. Scale bar= 100 µm. n = 4–6/group. * P < 0.05, * * P < 0.01.
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Fig. 6. Blood pressure, kidney function and histology in low-salt with candesartan with or without <t>L-NAME.</t> A. NO production in kidney tissue in 4 groups nephritis rats: normal-salt with candesartan (Dox+Can+NS), low-salt with candesartan (Dox+Can+LS), low-salt with candesartan and L-NAME (Dox+Can+LS+L- NAME), and normal-salt with candesartan and L-NAME (Dox+Can+NS +L-NAME). B-D. Blood pressure included SBP, DBP and MAP in the 4 groups. E-G. Kidney function index included SCr and BUN and calculated Ccr. H. Kidney histology changes in the 4 groups. The black arrowhead indicates glomerular shrinkage, and the yellow arrowhead indicates renal tubular necrosis. Scale bar= 100 µm. n = 4–6/group. * P < 0.05, * * P < 0.01.
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Fig. 6. Blood pressure, kidney function and histology in low-salt with candesartan with or without <t>L-NAME.</t> A. NO production in kidney tissue in 4 groups nephritis rats: normal-salt with candesartan (Dox+Can+NS), low-salt with candesartan (Dox+Can+LS), low-salt with candesartan and L-NAME (Dox+Can+LS+L- NAME), and normal-salt with candesartan and L-NAME (Dox+Can+NS +L-NAME). B-D. Blood pressure included SBP, DBP and MAP in the 4 groups. E-G. Kidney function index included SCr and BUN and calculated Ccr. H. Kidney histology changes in the 4 groups. The black arrowhead indicates glomerular shrinkage, and the yellow arrowhead indicates renal tubular necrosis. Scale bar= 100 µm. n = 4–6/group. * P < 0.05, * * P < 0.01.
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Fig. 6. Blood pressure, kidney function and histology in low-salt with candesartan with or without <t>L-NAME.</t> A. NO production in kidney tissue in 4 groups nephritis rats: normal-salt with candesartan (Dox+Can+NS), low-salt with candesartan (Dox+Can+LS), low-salt with candesartan and L-NAME (Dox+Can+LS+L- NAME), and normal-salt with candesartan and L-NAME (Dox+Can+NS +L-NAME). B-D. Blood pressure included SBP, DBP and MAP in the 4 groups. E-G. Kidney function index included SCr and BUN and calculated Ccr. H. Kidney histology changes in the 4 groups. The black arrowhead indicates glomerular shrinkage, and the yellow arrowhead indicates renal tubular necrosis. Scale bar= 100 µm. n = 4–6/group. * P < 0.05, * * P < 0.01.
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Fig. 6. Blood pressure, kidney function and histology in low-salt with candesartan with or without <t>L-NAME.</t> A. NO production in kidney tissue in 4 groups nephritis rats: normal-salt with candesartan (Dox+Can+NS), low-salt with candesartan (Dox+Can+LS), low-salt with candesartan and L-NAME (Dox+Can+LS+L- NAME), and normal-salt with candesartan and L-NAME (Dox+Can+NS +L-NAME). B-D. Blood pressure included SBP, DBP and MAP in the 4 groups. E-G. Kidney function index included SCr and BUN and calculated Ccr. H. Kidney histology changes in the 4 groups. The black arrowhead indicates glomerular shrinkage, and the yellow arrowhead indicates renal tubular necrosis. Scale bar= 100 µm. n = 4–6/group. * P < 0.05, * * P < 0.01.
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Fig. 6. Blood pressure, kidney function and histology in low-salt with candesartan with or without <t>L-NAME.</t> A. NO production in kidney tissue in 4 groups nephritis rats: normal-salt with candesartan (Dox+Can+NS), low-salt with candesartan (Dox+Can+LS), low-salt with candesartan and L-NAME (Dox+Can+LS+L- NAME), and normal-salt with candesartan and L-NAME (Dox+Can+NS +L-NAME). B-D. Blood pressure included SBP, DBP and MAP in the 4 groups. E-G. Kidney function index included SCr and BUN and calculated Ccr. H. Kidney histology changes in the 4 groups. The black arrowhead indicates glomerular shrinkage, and the yellow arrowhead indicates renal tubular necrosis. Scale bar= 100 µm. n = 4–6/group. * P < 0.05, * * P < 0.01.
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Fig. 6. Blood pressure, kidney function and histology in low-salt with candesartan with or without <t>L-NAME.</t> A. NO production in kidney tissue in 4 groups nephritis rats: normal-salt with candesartan (Dox+Can+NS), low-salt with candesartan (Dox+Can+LS), low-salt with candesartan and L-NAME (Dox+Can+LS+L- NAME), and normal-salt with candesartan and L-NAME (Dox+Can+NS +L-NAME). B-D. Blood pressure included SBP, DBP and MAP in the 4 groups. E-G. Kidney function index included SCr and BUN and calculated Ccr. H. Kidney histology changes in the 4 groups. The black arrowhead indicates glomerular shrinkage, and the yellow arrowhead indicates renal tubular necrosis. Scale bar= 100 µm. n = 4–6/group. * P < 0.05, * * P < 0.01.
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Fig. 6. Blood pressure, kidney function and histology in low-salt with candesartan with or without <t>L-NAME.</t> A. NO production in kidney tissue in 4 groups nephritis rats: normal-salt with candesartan (Dox+Can+NS), low-salt with candesartan (Dox+Can+LS), low-salt with candesartan and L-NAME (Dox+Can+LS+L- NAME), and normal-salt with candesartan and L-NAME (Dox+Can+NS +L-NAME). B-D. Blood pressure included SBP, DBP and MAP in the 4 groups. E-G. Kidney function index included SCr and BUN and calculated Ccr. H. Kidney histology changes in the 4 groups. The black arrowhead indicates glomerular shrinkage, and the yellow arrowhead indicates renal tubular necrosis. Scale bar= 100 µm. n = 4–6/group. * P < 0.05, * * P < 0.01.
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Chem Impex International nω nitro l arginine methyl ester
Fig. 6. Blood pressure, kidney function and histology in low-salt with candesartan with or without <t>L-NAME.</t> A. NO production in kidney tissue in 4 groups nephritis rats: normal-salt with candesartan (Dox+Can+NS), low-salt with candesartan (Dox+Can+LS), low-salt with candesartan and L-NAME (Dox+Can+LS+L- NAME), and normal-salt with candesartan and L-NAME (Dox+Can+NS +L-NAME). B-D. Blood pressure included SBP, DBP and MAP in the 4 groups. E-G. Kidney function index included SCr and BUN and calculated Ccr. H. Kidney histology changes in the 4 groups. The black arrowhead indicates glomerular shrinkage, and the yellow arrowhead indicates renal tubular necrosis. Scale bar= 100 µm. n = 4–6/group. * P < 0.05, * * P < 0.01.
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Fig. 6. Blood pressure, kidney function and histology in low-salt with candesartan with or without L-NAME. A. NO production in kidney tissue in 4 groups nephritis rats: normal-salt with candesartan (Dox+Can+NS), low-salt with candesartan (Dox+Can+LS), low-salt with candesartan and L-NAME (Dox+Can+LS+L- NAME), and normal-salt with candesartan and L-NAME (Dox+Can+NS +L-NAME). B-D. Blood pressure included SBP, DBP and MAP in the 4 groups. E-G. Kidney function index included SCr and BUN and calculated Ccr. H. Kidney histology changes in the 4 groups. The black arrowhead indicates glomerular shrinkage, and the yellow arrowhead indicates renal tubular necrosis. Scale bar= 100 µm. n = 4–6/group. * P < 0.05, * * P < 0.01.

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: A low-salt diet with candesartan administration is associated with acute kidney injury in nephritis by increasing nitric oxide.

doi: 10.1016/j.biopha.2023.114484

Figure Lengend Snippet: Fig. 6. Blood pressure, kidney function and histology in low-salt with candesartan with or without L-NAME. A. NO production in kidney tissue in 4 groups nephritis rats: normal-salt with candesartan (Dox+Can+NS), low-salt with candesartan (Dox+Can+LS), low-salt with candesartan and L-NAME (Dox+Can+LS+L- NAME), and normal-salt with candesartan and L-NAME (Dox+Can+NS +L-NAME). B-D. Blood pressure included SBP, DBP and MAP in the 4 groups. E-G. Kidney function index included SCr and BUN and calculated Ccr. H. Kidney histology changes in the 4 groups. The black arrowhead indicates glomerular shrinkage, and the yellow arrowhead indicates renal tubular necrosis. Scale bar= 100 µm. n = 4–6/group. * P < 0.05, * * P < 0.01.

Article Snippet: Both groups of rats were given 1 mg/kg/d candesartan i.p. NG-nitro-L-arginine methyl ester (L-NAME) (HY-18729A, MCE, USA, 10 mg/kg/d.ip), an inhibitor of endothelial nitric oxide synthase (eNOS), was intraperitoneally injected for 7 days, and the rats were divided into 4 groups: NS, LS, NS+L-NAME, and LS+L-NAME.

Techniques:

Fig. 7. Apoptosis detection in vivo and in vitro. The nephritis rats were divided into 4 groups, Dox+Can+NS (n = 4), Dox+Can+LS (n = 4), Dox+Can+LS+L- NAME (n = 6), and Dox+Can+NS +L-NAME (n = 6), and sacrificed on day 7. A-B. TUNEL staining and positive cell count in vivo. The brown nuclei indicated by the black arrows show TUNEL-positive cells, and the number per high-power field was greater in the low-salt treatment with candesartan but reversed by L-NAME and the eNOS inhibitor. Scale bar = 50 µm. C-E. western blotting and quantitative analysis of BAX, Bcl-2, and cleaved caspase-3 in vivo. F-H. HK-2 cells were pretreated with the NO donor SNP for different times, and BAX, Bcl-2, cleaved caspase-3 and Caspase-3 were analyzed by western blotting. n = 3–4/group. * P < 0.05, * * P < 0.01.

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: A low-salt diet with candesartan administration is associated with acute kidney injury in nephritis by increasing nitric oxide.

doi: 10.1016/j.biopha.2023.114484

Figure Lengend Snippet: Fig. 7. Apoptosis detection in vivo and in vitro. The nephritis rats were divided into 4 groups, Dox+Can+NS (n = 4), Dox+Can+LS (n = 4), Dox+Can+LS+L- NAME (n = 6), and Dox+Can+NS +L-NAME (n = 6), and sacrificed on day 7. A-B. TUNEL staining and positive cell count in vivo. The brown nuclei indicated by the black arrows show TUNEL-positive cells, and the number per high-power field was greater in the low-salt treatment with candesartan but reversed by L-NAME and the eNOS inhibitor. Scale bar = 50 µm. C-E. western blotting and quantitative analysis of BAX, Bcl-2, and cleaved caspase-3 in vivo. F-H. HK-2 cells were pretreated with the NO donor SNP for different times, and BAX, Bcl-2, cleaved caspase-3 and Caspase-3 were analyzed by western blotting. n = 3–4/group. * P < 0.05, * * P < 0.01.

Article Snippet: Both groups of rats were given 1 mg/kg/d candesartan i.p. NG-nitro-L-arginine methyl ester (L-NAME) (HY-18729A, MCE, USA, 10 mg/kg/d.ip), an inhibitor of endothelial nitric oxide synthase (eNOS), was intraperitoneally injected for 7 days, and the rats were divided into 4 groups: NS, LS, NS+L-NAME, and LS+L-NAME.

Techniques: In Vivo, In Vitro, TUNEL Assay, Staining, Cell Characterization, Western Blot